. The Canadian field-naturalist. Natural history; Sciences naturelles. 440 The Canadian Field-Naturalist Vol. 120. Barbed-wired enclosure Figure 2: Experimental design for Cougar detection, a passive method combining a baited hair-trap (central post and barbed-wired enclosure) protocol and a camera-trap (upper left comer, strapped on a tree). A volume of IS^iL contained 100-500 ng template DNA, Ix reaction buffer, 1.5 mmol (miUimoles) MgCl2, 0.3 mmol each primer, 250 mmol dNTPs and 1 U Taq polymerase. After initial incubation at 94°C for 2 min- utes, 40 cycles of PCR were performed at 94°C for
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. The Canadian field-naturalist. Natural history; Sciences naturelles. 440 The Canadian Field-Naturalist Vol. 120. Barbed-wired enclosure Figure 2: Experimental design for Cougar detection, a passive method combining a baited hair-trap (central post and barbed-wired enclosure) protocol and a camera-trap (upper left comer, strapped on a tree). A volume of IS^iL contained 100-500 ng template DNA, Ix reaction buffer, 1.5 mmol (miUimoles) MgCl2, 0.3 mmol each primer, 250 mmol dNTPs and 1 U Taq polymerase. After initial incubation at 94°C for 2 min- utes, 40 cycles of PCR were performed at 94°C for